penta mutant sortase a his6 (Addgene inc)
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penta mutant sortase a his6
Penta Mutant Sortase A His6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sortase+a+5m/pet30b-5M+SrtA+(Plasmid+%2351140)/10__7554_slash_elife__104302__3-316-26-41
Average 93 stars, based on 17 article reviews
Penta Mutant Sortase A His6, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sortase+a+5m/pet30b-5M+SrtA+(Plasmid+%2351140)/10__7554_slash_elife__104302__3-316-26-41
Average 93 stars, based on 17 article reviews
penta mutant sortase a his6 - by Bioz Stars,
2026-10
93/100 stars
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Clone Assay:Article Title: Mono- and Bi-specific Nanobodies Targeting the CUB Domains of PCPE-1 Reduce the Proteolytic Processing of Fibrillar Procollagens. Article Snippet: Periplasmic extracts were prepared by the osmotic shock method57 and soluble VHHs were purified by nickel-affinity chromatography (Qiagen) and SEC (Hiload 16/600 Superdex 75 pg; Cytiva) in 20 mM HEPES, 0.3 M NaCl pH 7.4. .. VHH-I5 and -H4 were also cloned for expression in the pHEN6 plasmid with a C-terminal Sortase recognition site (LPETG) and a 6-His tag (generous gift from Dr. Leo Hanke, Karolinska Institute, Stockholm, Sweden), and produced and purified as described in.59 In parallel, the Expressing:Article Title: Mono- and Bi-specific Nanobodies Targeting the CUB Domains of PCPE-1 Reduce the Proteolytic Processing of Fibrillar Procollagens. Article Snippet: Periplasmic extracts were prepared by the osmotic shock method57 and soluble VHHs were purified by nickel-affinity chromatography (Qiagen) and SEC (Hiload 16/600 Superdex 75 pg; Cytiva) in 20 mM HEPES, 0.3 M NaCl pH 7.4. .. VHH-I5 and -H4 were also cloned for expression in the pHEN6 plasmid with a C-terminal Sortase recognition site (LPETG) and a 6-His tag (generous gift from Dr. Leo Hanke, Karolinska Institute, Stockholm, Sweden), and produced and purified as described in.59 In parallel, the Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies. Article Snippet: .. Expression vectors and bacterial strains Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306, Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: Plasmid Preparation:Article Title: Mono- and Bi-specific Nanobodies Targeting the CUB Domains of PCPE-1 Reduce the Proteolytic Processing of Fibrillar Procollagens. Article Snippet: Periplasmic extracts were prepared by the osmotic shock method57 and soluble VHHs were purified by nickel-affinity chromatography (Qiagen) and SEC (Hiload 16/600 Superdex 75 pg; Cytiva) in 20 mM HEPES, 0.3 M NaCl pH 7.4. .. VHH-I5 and -H4 were also cloned for expression in the pHEN6 plasmid with a C-terminal Sortase recognition site (LPETG) and a 6-His tag (generous gift from Dr. Leo Hanke, Karolinska Institute, Stockholm, Sweden), and produced and purified as described in.59 In parallel, the Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies Article Snippet: .. Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306 , Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies. Article Snippet: .. Expression vectors and bacterial strains Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306, Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: Produced:Article Title: Mono- and Bi-specific Nanobodies Targeting the CUB Domains of PCPE-1 Reduce the Proteolytic Processing of Fibrillar Procollagens. Article Snippet: Periplasmic extracts were prepared by the osmotic shock method57 and soluble VHHs were purified by nickel-affinity chromatography (Qiagen) and SEC (Hiload 16/600 Superdex 75 pg; Cytiva) in 20 mM HEPES, 0.3 M NaCl pH 7.4. .. VHH-I5 and -H4 were also cloned for expression in the pHEN6 plasmid with a C-terminal Sortase recognition site (LPETG) and a 6-His tag (generous gift from Dr. Leo Hanke, Karolinska Institute, Stockholm, Sweden), and produced and purified as described in.59 In parallel, the Purification:Article Title: Mono- and Bi-specific Nanobodies Targeting the CUB Domains of PCPE-1 Reduce the Proteolytic Processing of Fibrillar Procollagens. Article Snippet: Periplasmic extracts were prepared by the osmotic shock method57 and soluble VHHs were purified by nickel-affinity chromatography (Qiagen) and SEC (Hiload 16/600 Superdex 75 pg; Cytiva) in 20 mM HEPES, 0.3 M NaCl pH 7.4. .. VHH-I5 and -H4 were also cloned for expression in the pHEN6 plasmid with a C-terminal Sortase recognition site (LPETG) and a 6-His tag (generous gift from Dr. Leo Hanke, Karolinska Institute, Stockholm, Sweden), and produced and purified as described in.59 In parallel, the Modification:Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies Article Snippet: .. Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306 , Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies. Article Snippet: .. Expression vectors and bacterial strains Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306, Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: Binding Assay:Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies Article Snippet: .. Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306 , Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: Article Title: Growth inhibition of Saccharomyces cerevisiae by SUMO-specific nanobodies. Article Snippet: .. Expression vectors and bacterial strains Cytoplasmic proteins were expressed in BL21 (DE3) cells using the following plasmid vectors: (1) pET SUMO vector and modified pET SUMO vectors: His6- Smt3p-Ube2G2 (smt3p: Uniprot ID Q12306, Ube2G2: Uniprot ID P60604 ), Smt3p-GFP-SBP-His6, Smt3p-Sortag motif (LPETG)-SBP-His6 (SBP: Streptavidin binding protein tag), (2) pET 28 vector: |